Immunoperoxidase staining

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Immunoperoxidase staining of leptospires.

Leptospires were detectable by light microscopy when Formalin-fixed preparations were reacted by direct and indirect methods with horseradish peroxidase-labeled specific antibody.

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Silver enhancement of polymerised diaminobenzidine: increased sensitivity for immunoperoxidase staining.

Unambiguous identification of lymphocytes is sometimes difficult because of weak immunostaining of the cell membrane immunoglobulins. A simple method of intensifying the diaminobenzidine (DAB) peroxidase reaction was therefore devised. Paraffin wax sections of formalin fixed tonsils and lymphomas were digested with trypsin and immunostained for kappa and lambda light immunoglobulin chains and C...

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Silver enhancement-of polymerised diaminobenzidine: Increased sensitivity for immunoperoxidase staining

Unambiguous identification of lymphocytes is sometimes difficult because of weak immunostaining of the cell membrane immunoglobulins. A simple method of intensifying the diaminobenzidine (DAB) peroxidase reaction was therefore devised. Paraffin wax sections of formalin fixed tonsils and lymphomas were digested with trypsin and immunostained for K and A light immunoglobulin chains and CD3 antige...

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Localization of plasminogen activators in human colon cancer by immunoperoxidase staining.

The immunoperoxidase technique, using antibodies against human urinary urokinase (Mr 55,000), was used for the localization of this enzyme in histological preparations of human colon tumors and normal colon tissue. The localization of tissue (vascular) activator was also investigated using antibodies against enzyme purified from human malignant melanoma. Both the "indirect method" and the perox...

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A quantitative study of the influence of fixation on immunoperoxidase staining of rectal mucosal plasma cells.

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 1980

ISSN: 0021-9746

DOI: 10.1136/jcp.33.12.1222-b